These two reports detail the completion of the development of quantitative Polymerase Chain Reaction (qPCR) assays to test for the presence of Perna viridis (PV) and Didemnum perlucidum (DP) in environmental DNA (eDNA) samples. The report outlines the protocols for establishing the limit of detection (LoD) for both the PV and DP assays, which involves creating serial dilutions of positive standards and analysing them via qPCR.The reports then present the results of the LoD analysis, including the confirmation of the LoD. Finally, the report includes the results of testing eDNA samples using the established assays.