https://oceanexpert.org/expert/Sethu

MSc

Research

Biological Oceanography, Marine Ecology
Chemical Oceanography

Seawater sampling and biogeochemical analysis; Identification of sea urchin gut microbiome:16S rRNA sequencing and NGS; Proteomics,Trace metal analysis of seawater

Arabian Sea

Molecular biology experience in DNA and RNA extractions from animal  tissues; cloning and protein expression; Protein extraction, SDS-PAGE and Western blotting, Immunohistochemistry, immunocytochemistry and microscopy (fluorescence and confocal microscope). In microbiology, isolation and purification of actinomycetes and other bacterial cultures. Bioinformatics data analysis of metagenomic sequencing-NGS-based 16S rRNA sequencing; analysis of phosphoproteomics data using Perseus software. Experience onboard cruises and other field trips (marine and freshwater), sample collection, and preservation of water, sediment and biological samples for various analyses. Analysis of different biogeochemical parameters in seawater including Dissolved oxygen, pH, Total organic carbon, Nutrients, Alkalinity, etc.; Trace metal analysis of seawater, sediments and biological samples using HR-ICP-MS. Experience in handling and sea urchins, dissections and microcosm experiments.

English